Development of Pharmaceutical substances based on blue-green alga Spirulina platensis

A blue-green alga S. platensis biomass is used as a basis for the development of pharmaceutical substances containing such vitally important trace elements as selenium, chromium and iodine. Using neutron activation analysis the possibility of target-oriented introduction of these elements into the S. platensis biocomplexes retaining its protein composition and natural beneficial properties has been proved. The curves of the dependence of the introduced element accumulation in the Spirulina biomass on its concentration in a nutrient medium, which make it possible to accurately measure out the required doses of the specified element in a substance, have been obtained. The peculiarities of interaction of various chromium forms (Cr(III) and Cr(VI) with the S. platensis biomass have been studied. It has been found that from a nutrient medium its cells mainly accumulate the vitally essential form of Cr(III) rather than toxic Cr(VI). Using the EPR technique and colorimetry, it has been demonstrated that the S. platensis substance enriched with Cr(III) is free from other toxic chromium forms. The developed technique can be used in pharmaceutical industry for the production of preparations containing Se, Cr, I, etc. on the basis of S. platensis biomass with the preservation of its natural beneficial properties and protein composition