The breeding of a wheat mutant pollen-derived variety Chuanfu No.5 and the related techniques

With the treatment of 150 Gy 60Co-γ irradiation to the dry F1 (Mianyang 88-334 x 8811525) hybrid seeds and the donor plants chosen from MF2, wheat anther culture was made based on MW14 and modified MS media and the pure diploid lines of MH1 derived from anther pollen were obtained. In 1996, the new mutant line 6086 and its sibling lines, 6086 and 6087, were selected and bred successfully. In 2002, the mutant pollen-derived line 6086 was denominated as Chuanfu No.5 by Sichuan Crop Variety Identification Committee and became the first mutant variety via anther culture of wheat in Sichuan. The success of Chuanfu No.5 shown that combining radiation induction and anther technique could shorten the breeding period and increase the efficiency of breeding of wheat