Stimulation of sewage sludge treatment by carbon sources and bioaugmentation with a sludge-derived microbial consortium

Highlights: • Sewage sludge disposal is one of the greatest challenges of the 21st century. • Wheat straw stabilized the SS better than faba bean straw and wood-chip pellets. • Sewage sludge treatment lead to an increase of Firmicutes, i.e., 32.70–53.84%. • The incubated non-amended sewage sludge showed an increase in virus abundance. • Bioaugmentation and amendments increased the yield of gene families GO ID. Recently, sewage sludge (SS) disposal has become one of the greatest global challenges. In this study, we aimed to evaluate the effect of faba bean straw (Straw-B), wheat straw (Straw-W), and wood-chip pellets (WCP) amended to SS, as well as bioaugmentation (BA), on the physicochemical characteristics and structure of the microbial community of the treated SS. Sixteen days of incubation of SS-containing mixtures revealed the highest efficiency of Straw-W(BA) in terms of SS stabilisation, i.e., the highest and most stable respiration intensity, the lowest ammonia emission, and the highest stimulation effect on the cress seedling growth. Shotgun sequencing data analysis showed that Proteobacteria dominated in the raw SS with 60.17% reads, which consisted of 16.40%, 29.18%, and 12.33% of Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria, respectively. All treated samples were characterised by an increased abundance of Firmicutes (32.70–53.84%). A remarkable increase in virus abundance (0.34% reads) was detected in the treated SS, which was incubated without C amendment and bioaugmentation. The addition of C sources to the SS changed some physicochemical characteristics of the mixture. All of these findings provide novel insights toward a mechanistic understanding of the fate of the human sewage microbiome in wastewater and other environments.