Published June 2011
| Version v1
Journal article
Implementation of 89Zr production and in vivo imaging of B-cells in mice with 89Zr-labeled anti-B-cell antibodies by small animal PET/CT
Creators
- 1. Institute of Radiopharmacy, Helmholtz-Zentrum Dresden-Rossendorf, P.O. Box 510 119, 01314 Dresden (Germany)
- 2. Leibniz-Institute for Natural Products Research and Infection Biology, Hans-Knoell-Institute, Jena (Germany)
- 3. Institute of Immunology, University Hospital Jena, Jena (Germany)
- 4. PINSTECH/IPD P.O. Nilore Islamabad (Pakistan)
Description
We examined the production, separation, and characterization of 89Zr, including supplementation of a commercial Cyclone 18/9 with a self-made Solid Target System (STS). Obtained [89Zr]Zr-oxalate was used for the labeling of anti-B cell antibodies with desferrioxamine-p-SCN as a bifunctional chelator. 89Zr-labeled antibodies were injected in DBA/1 mice to examine usability for detection of B cells in vivo. PET measurements showed binding of 89Zr-labeled anti-B cell antibodies in tissues with high frequencies of B cells, i.e. in spleen and lymph nodes.
Availability note (English)
Available from http://dx.doi.org/10.1016/j.apradiso.2011.02.040Additional details
Identifiers
- DOI
- 10.1016/j.apradiso.2011.02.040;
- PII
- S0969-8043(11)00133-3;
Publishing Information
- Journal Title
- Applied Radiation and Isotopes
- Journal Volume
- 69
- Journal Issue
- 6
- Journal Page Range
- p. 852-857
- ISSN
- 0969-8043
- CODEN
- ARISEF
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 42077133
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S07: ISOTOPES AND RADIATION SOURCES;
- Descriptors DEI
- IN VIVO; ISOTOPE PRODUCTION; LABELLING; LYMPH NODES; MICE; MONOCLONAL ANTIBODIES; OXALATES; POSITRON COMPUTED TOMOGRAPHY; SPLEEN; ZIRCONIUM 89
- Descriptors DEC
- ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACID SALTS; COMPUTERIZED TOMOGRAPHY; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; ELECTRON CAPTURE RADIOISOTOPES; EMISSION COMPUTED TOMOGRAPHY; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LYMPHATIC SYSTEM; MAMMALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ORGANS; RADIOISOTOPES; RODENTS; TOMOGRAPHY; VERTEBRATES; ZIRCONIUM ISOTOPES
Optional Information
- Copyright
- Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.