Published February 16, 1987 | Version v1
Journal article Metadata-only

Preparation and characterization of reagents for epitestosterone radioimmunoassay

Creators

  • 1. Vyzkumny Ustav Endokrinologicky, Prague (Czechoslovakia)
  • 2. Institute of Clinical Endocrinology, Lubochna (Czechoslovakia)

Description

The preparation of reagents, suitable for radioimmunoassay of 17α-hydroxy-4-androsten-3-one (epitestosterone) in body fluids is described. It includes a simplified synthesis of the immunogen, epitestosterone-3-(0-carboxymethyl)oxime coupled to bovine serumalbumin its characterization and preparation of the radioligand (125I) iodohistaminyl derivative of epitestosterone. The assay protocol for epitestosterone detection in urine avoiding extraction is described and the reliability criteria of the method are given. (author)

Additional details

Publishing Information

Journal Title
J. Radioanal. Nucl. Chem.
Journal Volume
108
Journal Issue
3
Series
J. Radioanal. Nucl. Chem. ;Letters.
Journal Page Range
159-168
ISSN
0236-5731
CODEN
JRNCD

INIS

Country of Publication
Hungary
Country of Input or Organization
Hungary
INIS RN
18064214
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ACCURACY; CHEMICAL PREPARATION; EXCRETION; IODINE 125; LABELLED COMPOUNDS; METABOLISM; RADIOIMMUNOASSAY; RADIOIMMUNODETECTION; SAMPLE PREPARATION; STEROIDS; TESTOSTERONE; URINE
Descriptors DEC
ANDROGENS; ANDROSTANES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CLEARANCE; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; STEROID HORMONES; SYNTHESIS; TRACER TECHNIQUES; WASTES

Optional Information

Notes
12 refs.; 2 tables.