Synthesis and in vitro Antioxidant Activity of chloro-substituted Hydrazone

Hydrazones have been reported for various biological activities, including their scavenging radical activity as antioxidants. The presence of azomethine group (-NHN=CH-) makes this compound one of the important classes in many synthetic products. In this study, ortho and meta-positions of chloro-substituted hydrazones have been synthesized through condensation reaction between substituted benzaldehydes and phenylhydrazine under microwave irradiation. The structure of the synthesized compounds was confirmed by UV, FTIR, ¹H-NMR, and GC-MS spectroscopic data while antioxidant activity was tested by the DPPH method. Hydrazone compounds were successfully synthesized in good yield and short-time reaction. The results of in vitro antioxidant activity assay showed that chlorohydrazone have very good antioxidant activity, whereas meta has better activity than ortho-position with IC₅₀ value of 25.8 and 57 µg/mL respectively. The results demonstrated that these active compounds may use to support as a decent stand for further investigation in a way to ascertain innovative antioxidant medicines. (paper)