Published May 1994 | Version v1
Journal article Metadata-only

Development of a radioreceptor assay for β2 adrenergic agonists

Creators

  • 1. Lab. d'analyse des denrees alimentaires d'origine animale, Faculte de Medecine Veterinaire de l'Universite, Liege (Belgium)

Description

Several β2 adrenergic agonists are illegally used as growth promoters in meat production. We have developed and evaluated a radioreceptor assay for the multianalyte detection of these compounds. The method is based on a competition for binding with receptors (plasma membranes prepared from bovine teat muscles) between a radioactive tracer (3H-dihydroalprenolol) and β2 agonist residues present in the samples. The method has been validated for three β2 agonists (clenbuterol, mabuterol and cimaterol) in bovine urine samples. The detection limit (mean of ''blank'' values + 3 SEM) in urine was 2.4 ppb clenbuterol. Using this procedure, samples containing at least 5 ppb of clenbuterol, mabuterol or cimaterol could be identified as positive for the presence of β2 agonists. (orig.)

Abstract (German)

Mehrere β2 adrenerge Agonisten werden illegal als Wachstumsfoerderer in der Fleischproduktion eingesetzt. Wir entwickelten und testeten einen RRA (''Radioreceptor Assay'') zur Mehrfachrueckstandsanalyse dieser Zusammensetzungen. Die Methode basiert auf einer Kompetition eines radioaktiven Markers (3H-dihydroalpenolol) mit den Rueckstaenden der β2 Agonisten der Proben um Bindungsstellen der Rezeptoren (Plasmamembranen, welche aus Muskelzellen von Rinderzitzen gewonnen wurden). Die Methode wurde fuer 3 β2 Agonisten (Clenbuterol, Mabuterol und Cimaterol) in Harnproben anerkannt. Die Nachweisgrenze (Durchschnitt der Leerwerte + 3 Standardabweichungen) bei Harnproben liegt bei 2,4 ppb fuer Clenbuterol. Diese Methode ermoeglicht, Konzentrationen von mindestens 5 ppb an Clenbuterol, Mabuterol und Cimaterol im Probenmaterial nachzuweisen. (orig.)

Additional details

Publishing Information

Journal Title
Archiv fuer Lebensmittelhygiene
Journal Volume
45
Journal Issue
3
Journal Page Range
p. 57-61.
ISSN
0003-925X
CODEN
ALMHAO

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
27047370
Subject category
S60: APPLIED LIFE SCIENCES; S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ADRENALINE; CELL MEMBRANES; DOMESTIC ANIMALS; GROWTH; HORMONES; MEAT; MUSCLES; RADIORECEPTOR ASSAY; STIMULATION; TRITIUM
Descriptors DEC
ADRENAL HORMONES; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARDIOTONICS; CARDIOVASCULAR AGENTS; CELL CONSTITUENTS; DRUGS; FOOD; HYDROGEN ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MEMBRANES; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; RADIOASSAY; RADIOISOTOPES; SYMPATHOMIMETICS; TRACER TECHNIQUES; YEARS LIVING RADIOISOTOPES