Studies on the quality control of insulin radioimmunoassay kit pt. 1

A typical abnormal standard dose response curve of nearly convex form often encountered in insulin radioimmunoassay (IRIA) has been analyzed by varying incubation conditions. The cause of such abnormality has been turned out to be the results of an incomplete equilibrium between the two reactants. By careful control of the temperature of serum sample an immediate cause of sharp deviation of B/F value of the sample tube from the measurable range in the standard curve has also been investigated. The two main troubles have been proven to be stemmed from incubation conditions. Incubation at 40C for 48 hrs is emphasized for IRIA. (author)