Published 1984
| Version v1
Journal article
Radioimmunoassay for free and bound forms of abscisic acid
- 1. Natal Univ., Pietermaritzburg (South Africa). Dept. of Horticultural Science
- 2. Natal Univ., Pietermaritzburg (South Africa). Dept. of Animal Science
Description
A radioimmunoassay (RIA) for the quanitation of abscisic acid (ABA) has been developed. The assay is extremely sensitive and measuring ranges extend from 10 pg to 10 ng. Although the assay was free of contaminant interference when applied to avocado material, crude extract analysis yielded a composite of free and bound forms of ABA. Equivalents of 20 mg of plant material were spotted onto silica gel plates (GF245 solvent:toluene:ethyl acetate : acetic acid 25:15:3), developed and the relative Rf zones removed and subjected to RIA. The technique was tested on avocados
Additional details
Publishing Information
- Journal Title
- Crop Prod.
- Journal Volume
- 13
- Series
- Crop Prod.
- Journal Page Range
- 38
- ISSN
- 0376-4567
INIS
- Country of Publication
- South Africa
- Country of Input or Organization
- South Africa
- INIS RN
- 16026757
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AVOCADOS; HORMONES; PLANT GROWTH; RADIOIMMUNOASSAY; SENSITIVITY; THIN-LAYER CHROMATOGRAPHY
- Descriptors DEC
- CHROMATOGRAPHY; FOOD; FRUITS; GROWTH; ISOTOPE APPLICATIONS; SEPARATION PROCESSES; TRACER TECHNIQUES