Published 1977 | Version v1
Journal article Metadata-only

Thyrotropin-releasing hormone radioimmunoassay

Creators

  • 1. Centre de Lutte contre le Cancer Georges-Francois-Leclerc, 21 - Dijon (France)

Description

The preliminary steps for a radio-immunoassay of Thyrotropin Releasing Hormone (TRH) are described. Rabbit antiserum at dilution 1:10000 is used with radioiodinated TRH (125I). It is possible to assay from 5 to 1000 pg unlabeled TRH with an intraassay reproducibility varying from 7 to 4% and the lowest detectable amount in this system is 10 pg TRH. TRH mean and standard deviation in normal subjects are 136,9 and 52,9 pg/ml

Abstract (French)

On a decrit les resultats preliminaires d'un dosage radioimmunologique de la Thyrotropin Releasing Hormone (TRH). On a utilise un antiserum de Lapin a la dilution 1:10000 avec de la TRH marquee a l'iode 125. L'etendue de la gamme de dosage couvre l'intervalle de 5 a 1000 pg de TRH froide. La reproductibilite intradosage varie entre 7 et 4% et la sensibilite du systeme est de 10 pg. La moyenne et l'ecart-type obtenus avec un echantillon de 72 sujets normaux sont respectivement de 136,9 et 52,9 pg/ml

Additional details

Additional titles

Original title (French)
Dosage radioimmunologique de la thyrotropin releasing hormone

Publishing Information

Journal Title
C. R. Seances Soc. Biol. Fil.
Journal Volume
171
Journal Issue
6
Series
C. R. Seances Soc. Biol. Fil.
Journal Page Range
1216-1220

INIS

Country of Publication
France
Country of Input or Organization
France
INIS RN
10433109
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
IODINE 125; RADIOIMMUNOASSAY; TRH
Descriptors DEC
BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; RADIOISOTOPES; TRACER TECHNIQUES