The study on labelling conditions and in vitro stability of 186Re-labelled anti-human brain glioma monoclonal antibody SZ-39

Monoclonal Antibody can be directly labelled with 186Re or 188Re. Using anti-human brain glioma monoclonal antibody (SZ-39) as a model, the authors studied some factors that influence labelling efficiency, such as pH of reaction mixture, various trans-chelation agents (sodium tartrate, sodium citrate, sodium gluconate), the labelling time and reduction agents (AA, DTT, 2-ME) for reducing disulfide groups in SZ39. At last, the authors established a better labelling method. The optimum pH was showed to be 2.54, sodium gluconate was used as trans-chelation agent. The labelling efficiency as determined by thin-layer chromatography and HPLC analysis was 96.2%. The labelled SZ39 was tested by quantitative HPLC to determine the radiochemical purity and subsequent purification was collected for testing stability in vitro. The labelled SZ39 has high radiochemical purity and was stable in 4 degree C when BSA was added