Sequence Analysis of the Carotenoid Isomerase Gene in Potato (Solanum tuberosum)

Carotenoid Isomerase (CRTISO) is an important enzyme in carotenoid biosynthesis. Here, the Solanum tuberosum CRTISO (StCRTISO) gene sequences were obtained from Spud DB database, and preformed for sequence analysis. The StCRTISO gene mapped to chromosomes 10, and contains an open reading frame of 1,848 bp that encodes a 615-amino acid protein with a calculated molecular mass of 67.52 kD and an isoelectric point (pI) of 6.92. Subcellular localization predicted the StCRTISO gene was in the cytoplasm. The conserved domain of the StCRTISO protein is Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The StCRTISO protein is most closely related to Solanum lycopersicum. The findings of the present study provide a molecular basis for the elucidation of CRTISO gene function in potato. (paper)