Role of nitrogen and phosphate dinamics to increase plant survival grown on oil contaminated soil

The petroleum mining industry plays an important role in the fulfillment of human needs but also has a negative impact on less productive soil due to oil contamination that causes soil nutrient degradation such as nitrogen and phosphate level. This research aims to study the dynamics of nitrogen and phosphate on oil-contaminated soil by utilizing multisymbiotic soil bacteria (Pseudomonas sp. and Rhizobium sp.) as an effort to improve the quality of nitrogen and phosphate availability that affect plant resistance to survive in the oil-contaminated soil. The research method was Randomized Complete Design with soil bacterial treatments (Pseudomonas sp. and Rhizobium sp.), consisting of 3 treatment i.e (1) Pseudomonas sp., (2) Rhizobium sp., and (3) combination of Pseudomonas sp. and Rhizobium sp. Each treatment at a concentration of 10⁸ cells/gram with 4 repetitions. Data was obtained including Total Petroleum Hydrocarbon (TPH), P, C, N concentration in soil, plant growth and environmental parameters including temperature, pH, and soil moisture. Analysis of the data used one way variance analysis followed by LSD test. The results showed that plant survival on oil-contaminated soils is not only determined by the dynamics of nitrogen and phosphate availability but also by the availability of TPH content in the soil that indicated by optimal results when there is a multisymbiotic role of both bacteria that inserted into the soil. (paper)