Published May 2004 | Version v1
Journal article

Effects of quercetin on release and synthesis of ET-1 and NO of HUVEC in hypoxia

  • 1. Suzhou Univ., Suzhou (China). Medical School, Dept. of Pharmacology

Description

Objective: To investigate the protective effects of quercetin (Que) on endothelial cells from hypoxia. Methods: Human umbilical vein cells (HUVEC) were cultured in vitro and hypoxia was applied to induce damage of HUVEC. Radioimmunoassay (RIA) and colorimetry were used to assess the amounts of ET-1 and NO in the supernatant of normal HUVEC, or HUVEC damaged by hypoxia. Results: Following the treatment of the cultured HUVEC with hypoxia for 48 h, the level of NO in the supernatant was decreased while that of ET-1 was increased. Pretreatment of HUVEC with Que (10, 20, 40, 80 μmol/L), could significantly attenuate these changes. Conclusion: Que can increase the concentration of NO and decrease that of ET-1 released by HUVEC during hypoxia, which could be the possible mechanism of its protection on HUVEC

Additional details

Additional titles

Augmented title (English)
The HUVEC stands for Human Umbilical Vein Cells

Publishing Information

Journal Title
Acta Academiae Medicinae Suzhou
Journal Volume
24
Journal Issue
3
Journal Page Range
p. 280-282
ISSN
1000-5749