Effects of quercetin on release and synthesis of ET-1 and NO of HUVEC in hypoxia
Creators
- 1. Suzhou Univ., Suzhou (China). Medical School, Dept. of Pharmacology
Description
Objective: To investigate the protective effects of quercetin (Que) on endothelial cells from hypoxia. Methods: Human umbilical vein cells (HUVEC) were cultured in vitro and hypoxia was applied to induce damage of HUVEC. Radioimmunoassay (RIA) and colorimetry were used to assess the amounts of ET-1 and NO in the supernatant of normal HUVEC, or HUVEC damaged by hypoxia. Results: Following the treatment of the cultured HUVEC with hypoxia for 48 h, the level of NO in the supernatant was decreased while that of ET-1 was increased. Pretreatment of HUVEC with Que (10, 20, 40, 80 μmol/L), could significantly attenuate these changes. Conclusion: Que can increase the concentration of NO and decrease that of ET-1 released by HUVEC during hypoxia, which could be the possible mechanism of its protection on HUVEC
Additional details
Additional titles
- Augmented title (English)
- The HUVEC stands for Human Umbilical Vein Cells
Publishing Information
- Journal Title
- Acta Academiae Medicinae Suzhou
- Journal Volume
- 24
- Journal Issue
- 3
- Journal Page Range
- p. 280-282
- ISSN
- 1000-5749
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 36029081
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Quality check status
- Yes
- Descriptors DEI
- ANOXIA; BIOLOGICAL FUNCTIONS; DIAGNOSIS; ENDOTHELINS; MEDICAL EXAMINATIONS; MEDICAL SURVEILLANCE; NITRIC OXIDE; PATIENTS; POLYPEPTIDES; QUERCETIN; RADIOIMMUNOASSAY; THERAPY; VEINS;
- Descriptors DEC
- AROMATICS; BIOASSAY; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CHALCOGENIDES; DIAGNOSTIC TECHNIQUES; FLAVONES; FLAVONOIDS; HETEROCYCLIC COMPOUNDS; HETEROCYCLIC OXYGEN COMPOUNDS; HYDROXY COMPOUNDS; IMMUNOASSAY; ISOTOPE APPLICATIONS; MEDICAL SURVEILLANCE; MEDICINE; NITROGEN COMPOUNDS; NITROGEN OXIDES; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANS; OXIDES; OXYGEN COMPOUNDS; PEPTIDES; PHENOLS; POLYPEPTIDES; POLYPHENOLS; PROTEINS; PYRANS; RADIOASSAY; RADIOIMMUNODETECTION; TRACER TECHNIQUES;