Published October 9, 2009 | Version v1
Journal article Metadata-only

Lycopene inhibits PDGF-BB-induced retinal pigment epithelial cell migration by suppression of PI3K/Akt and MAPK pathways

Creators

  • 1. Department of Ophthalmology, Cardinal Tien Hospital, Taipei Hsien, Taiwan, ROC (China)
  • 2. School of Medicine, Fu Jen Catholic University, Taipei Hsien, Taiwan, ROC (China)
  • 3. Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan, ROC (China)
  • 4. Department of Biotechnology, Vanung University, Taoyuan, Taiwan, ROC (China)

Description

Retinal pigment epithelial (RPE) cells play a dominant role in the development of proliferative vitreoretinopathy (PVR), which is the leading cause of failure in retinal reattachment surgery. Several studies have shown that platelet-derived growth factor (PDGF) exhibits chemotaxis and proliferation effects on RPE cells in PVR. In this study, the inhibitory effect of lycopene on PDGF-BB-induced ARPE19 cell migration is examined. In electric cell-substrate impedance sensing (ECIS) and Transwell migration assays, significant suppression of PDGF-BB-induced ARPE19 cell migration by lycopene is observed. Cell viability assays show no cytotoxicity of lycopene on RPE cells. Lycopene shows no effect on ARPE19 cell adhesion and is found to inhibit PDGF-BB-induced tyrosine phosphorylation and the underlying signaling pathways of PI3K, Akt, ERK and p38 activation. However, PDGF-BB and lycopene show no effects on JNK activation. Taken together, our results demonstrate that lycopene inhibits PDGF-BB-induced ARPE19 cell migration through inhibition of PI3K/Akt, ERK and p38 activation.

Availability note (English)

Available from http://dx.doi.org/10.1016/j.bbrc.2009.07.155

Additional details

Identifiers

DOI
10.1016/j.bbrc.2009.07.155;
PII
S0006-291X(09)01554-X;

Publishing Information

Journal Title
Biochemical and Biophysical Research Communications
Journal Volume
388
Journal Issue
1
Journal Page Range
p. 172-176
ISSN
0006-291X
CODEN
BBRCA9

INIS

Country of Publication
United States
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
45020657
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
GROWTH FACTORS; INHIBITION; PHOSPHORYLATION; RHODOPSIN; SUBSTRATES; SURGERY; TOXICITY; TYROSINE
Descriptors DEC
AMINO ACIDS; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; HYDROXY ACIDS; MEDICINE; MITOGENS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PIGMENTS; PROTEINS

Optional Information

Copyright
Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.