Effect of microwave- and ionizing radiation on activity of Na-K-ATPase in Ehrlich ascites carcinoma cells

The levels of Na-K-ATPase were measured in a microsome fraction of Ehrlich ascites carcinoma cells immediately, 10 and 30 min after exposure to microwave (series 1), 7 Gy of gamma-radiation (series 2) and different sequences of complex application of the agents (UHF+gamma-radiation) (series 3), (gamma-radiation+microwave) (series 4). Immediately after exposure, enzyme activity showed a significant decrease in series 1 and 4 (ser. 1-64 and ser. 4-58% as compared with control, α<0.05). A similar tendency was observed in series 2 and 3 (ser. 2-76 and ser. 3-84% as compared with control, α<0.05). With further exposure, it remained prcatically unchaged in ser. 1 and 4; in ser. 2 and 3 it continued to decrease within 10 min (ser. 2-42 2-49 and ser. 3-69%, α<0.05). The most pronounced inactivation was observed after exposure to gamma rays. Application of the latter in conjunction with microwave-field heating was folloed by a protective effect