Study of the radioprotective efficiency of combined administration of natural antioxidants and a sulfhydryl compound in feverish irradiated rats

In the present experiments, a study of the radioprotective effects of natural antioxidants, rutin alone, vitamine E alone or each of them combined with synthetic radioprotector, cysteine have been investigated in irradiated and feverish irradiated rats. Furthermore, the oxidative stress bio markers and certain liver function tests of the irradiated and the feverish whole body irradiated rats were examined. Two main sets of animals were used: The 1st set was constructed in order to study the effect of irradiation, while the second set was used to study the effect of irradiation on feverish rats. The effect of irradiation was evaluated by exposing the whole body of rats to gamma radiation at acute single dose level of 6.5 Gy. Rutin was orally daily administered for two weeks before irradiation in a dose of 1.064 mmol/kg , vitamine E was injected intraperitoneally daily for seven days before irradiation in a dose of 50 mg/100 g. While, cysteine was intraperitoneally administered only 30 min. before irradiation in a dose of 30 mg/kg. In order to determine the antipyretic effect of the drugs, body temperature of each animal was measured before induction of hyperthermia as well as 18 hours following yeast injection. Rats were treated with the tested drugs before induction of fever then exposed to whole body gamma radiation at acute single dose level of 6.5 Gy and body temperature of each animal was measured 3 days after irradiation