99mTc-Luteolin: Radiolabeling, In Silico ADMET and Biological Evaluation as a Natural Tracer Tumor imaging
Creators
- 1. Labeled Compounds Department, Hot Lab. Center, Egyptian Atomic Energy Authority (EAEA), Cairo (Egypt)
- 2. Cyclotron Project, Nuclear Research Centre, Cairo (Egypt)
- 3. Departement of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Nahda University (NUB), Beni-Sweif (Egypt)
- 4. Radioactive Isotopes and Generators Department, Hot Lab. Center, Egyptian Atomic Energy Authority (EAEA), Cairo (Egypt)
- 5. Biology Department, Nuclear Research Center, Egyptian Atomic Energy Authority (EAEA), Cairo (Egypt)
- 6. Drug Radiation Research Department, National Center for Radiation Research and Technology Atomic Energy Authority, Cairo (Egypt)
- 7. Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef (Egypt)
- 8. Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef (Egypt)
Description
The focus of the staged work was radiolabelled 5,7,3', 4' tetra-hydroxy flavone (Luteolin), isolated from Plantago lanceolata L., (F. Plantaginaceae), with 99mtechnetium (99mTc) to produce a natural tracer 99mTc-Luteolin (99mTc-Lut), and to evaluate the biological distribution in silico and in vivo. Labeling was carried out by reduction reaction using SnCl2 as reducing agent giving a maximum radiochemical yield (RCY) up to 97% at 40 degree C, ph4, for 30 min., with concentration of 99mTc and Luteolin of 150 muL. The tracer (99mTc-Lut) showed 12 h., in vitro stability. In silico ADMET screening of 99mTc-Lut indicated its high bioavailability in all body organs. The bio-distribution profile indicating the highly uptake of the tracer 99mTc-Lut, in tumor mice target/non-target ratio (T/NT) up to two-fold compared to normal mice, whereas the clearance from mice was observed via both renal and feces pathway. 9'9mTc-Lut, could be valued as a probable natural radiopharmaceutical tracer for tumor appearance
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Research and Applied Sciences
- Journal Volume
- 14
- Journal Issue
- 1
- Journal Page Range
- p. 125-132
- ISSN
- 1687-8507
INIS
- Country of Publication
- Egypt
- Country of Input or Organization
- Egypt
- INIS RN
- 54000231
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BLOOD; FLAVONOIDS; LIVER CELLS; MICE; NEOPLASMS; RADIOCHEMISTRY; RENAL CLEARANCE; TECHNETIUM 99; URINE
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CHEMISTRY; CLEARANCE; DISEASES; EXCRETION; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; TECHNETIUM ISOTOPES; VERTEBRATES; WASTES; YEARS LIVING RADIOISOTOPES