Published January 15, 1979
| Version v1
Journal article
An estimate of the minimal frequency of cytolytic T lymphocyte effector cells generated in allogeneic reactions
Creators
- 1. Institut Suisse de Recherches Experimentales sur le Cancer, Lausanne
- 2. Chicago Univ., IL (USA). Dept. of Pharmacology
Description
A 'micro' modification of the standard 51Cr release assay was developed. With this assay, significant cytolysis was obtained employing 100-200 51Cr-labeled target cells and fewer that 100 lymphocytes obtained from mixed leukocyte cultures. A minimal estimate as to the actual frequency of effector cells was determined for both primary (1 cell in 30) and secondary (1 cell in 8) cytolytic T lymphocyte population. (Auth.)
Additional details
Publishing Information
- Journal Title
- J. Immunol. Methods
- Journal Volume
- 25
- Journal Issue
- 1
- Series
- J. Immunol. Methods.
- Journal Page Range
- 13-20
- ISSN
- 0022-1759
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 10452234
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CHROMIUM 51; CYTOLOGICAL TECHNIQUES; LABELLED COMPOUNDS; LYMPHOCYTES; MICE; RADIOASSAY
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHROMIUM ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOTOPES; LEUKOCYTES; MAMMALS; NUCLEI; RADIOISOTOPES; RODENTS; SOMATIC CELLS; VERTEBRATES