Optimized DNA-based bioassay for Leptospira interrogans detection: a novel platform for leptospirosis diagnosis
Creators
- 1. Shoolini University, Faculty of Applied Sciences and Biotechnology (India)
- 2. CSK HP Agriculture University, DGCN College of Veterinary and Animal Sciences (India)
- 3. Postgraduate Institute of Medical Education and Research (India)
Description
An optimized DNA-based bioassay for Leptospira interrogans detection has been developed. Electrochemical studies of the developed biosensor were done using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Surface characterization of the biosensor was done using scanning electron microscopy (SEM). The biosensor showed specificity to L. interrogans as determined by specificity studies. The sensitivity of the biosensor was 264.5 µA/cm2/ng and lower limit of detection (LOD) was 0.015 ng/6 µl using CV. The biosensor was also validated with serum samples spiked with single-stranded leptospiral DNA. The developed biosensor also showed good stability for a period of 6 months at 4 °C as shown by the DPV analysis.;
Research highlights
The developed DNA-based bioassay is the first report on biosensor-based leptospirosis diagnosis.The developed bioassay is a DNA-based amperometric biosensor specific to L. interrogans causing leptospirosis.
The biosensor has a sensitivity of 264.5 µA/cm2/ng and a lower limit of detection (LOD) of 0.015 ng/6µl.
The biosensor was stable for 6 months with only 13% loss in DPV peak current.
Additional details
Identifiers
Publishing Information
- Journal Title
- 3 Biotech
- Journal Volume
- 9
- Journal Issue
- 7
- Journal Page Range
- p. 1-7
- ISSN
- 2190-5738
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 51085326
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AMPEROMETRY; BIOASSAY; DIAGNOSIS; DNA; ELECTROCHEMISTRY; SCANNING ELECTRON MICROSCOPY; SPECIFICITY; VOLTAMETRY
- Descriptors DEC
- CHEMICAL ANALYSIS; CHEMISTRY; ELECTRON MICROSCOPY; MICROSCOPY; NUCLEIC ACIDS; ORGANIC COMPOUNDS; QUANTITATIVE CHEMICAL ANALYSIS; TITRATION; VOLUMETRIC ANALYSIS
Optional Information
- Copyright
- Copyright (c) 2019 King Abdulaziz City for Science and Technology