Effect of angiotensin II on cell proliferation and collagen synthesis following 60Co irradiation
Creators
- 1. Institute of Radiation Medicine, Beijing (China)
- 2. Institute of Toxicant and Drug, Beijing (China)
Description
Objective: To explore the mechanism of 60Co irradiation on cellular proliferation and synthesis of type I pro-collagen protein in cultured human embryo lung fibroblasts (HELF). Method: Cultured cells were divided into groups irradiated with different doses plus angiotensin II (A II) stimulation. Relationship of malondialdehyde (MDA) content in medium and extent of cellular proliferation were measured, immuno-reactive material of intracellular A II and type I pro-collagen protein were displayed by method of immunocytochemistry, and image pattern analysis was made. Results: Irradiation with 1-5 Gy could stimulate cell proliferation and synthesis of type I pro-collagen significantly; cell proliferation and type I pro-collagen synthesis could be promoted by exogenous A II. Conclusion: Irradiation can stimulate HELF to synthesis A II, which stimulates cell proliferation and type I pro-collagen synthesis. It may be related to HELF proliferation and collagen synthesis induced by irradiation
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 18
- Journal Issue
- 2
- Journal Page Range
- p. 98-100
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 29049771
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ANGIOTENSIN; BIOLOGICAL RADIATION EFFECTS; BIOSYNTHESIS; CELL CULTURES; CELL PROLIFERATION; CHEST; COBALT 60; COLLAGEN; DOSE-RESPONSE RELATIONSHIPS; EMBRYOS; FIBROBLASTS; GAMMA RADIATION; LUNGS; RADIATION DOSES
- Descriptors DEC
- ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BODY; BODY AREAS; CARDIOVASCULAR AGENTS; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; DRUGS; ELECTROMAGNETIC RADIATION; GLOBULINS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RESPIRATORY SYSTEM; SCLEROPROTEINS; SOMATIC CELLS; SYNTHESIS; VASOCONSTRICTORS; YEARS LIVING RADIOISOTOPES