Published 1995
| Version v1
Miscellaneous
DNA polymorphism detection on rice mutant lines by two short oligonucleotides
- 1. Malaysian Institute for Nuclear Technology Research (MINT), Bangi (Malaysia). Agro Technology and Bioscience Div.
Description
Three gamma irradiation induced-mutants of Oryza sativa, MA03, B1 and B11, which were selected from a population segregating for semi-dwarfism, were subjected to RAPD analysis and compared to the parent line MR52. DNA fragments were amplified by combinations of two arbitrary oligonucleotide primers of 10 bp in length each. A total of 20 arbitrary primers were used for the pair combinations. Majority of the amplified fragments were common to all mutant lines and the parent line. Four pairs of primers combination produced amplified DNA fragments which were common to all three mutant lines. These fragments have been identified as having potential to be used as a molecular marker for certain locus of the mutant lines
Additional details
Publishing Information
- Imprint Title
- Compilation of research papers 1994
- Imprint Pagination
- 671 p.
- Journal Page Range
- p. 411-418.
- Report number
- INIS-mf--14786
INIS
- Country of Publication
- Malaysia
- Country of Input or Organization
- Malaysia
- INIS RN
- 27050687
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- DNA POLYMERASES; GAMMA RADIATION; GENE AMPLIFICATION; RADIATION INDUCED MUTANTS; RICE
- Descriptors DEC
- CEREALS; ELECTROMAGNETIC RADIATION; ENZYMES; GRAMINEAE; IONIZING RADIATIONS; LILIOPSIDA; MAGNOLIOPHYTA; MUTANTS; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PLANTS; POLYMERASES; PROTEINS; RADIATIONS; TRANSFERASES