Fluorescence detection of dopamine based on the peroxidase-like activity of Fe₃O₄-MWCNTs@Hemin

A novel Fe₃O₄-MWCNTs@Hemin nanocomposite was synthesized using hemin and Fe₃O₄ with multi-walled carbon nanotubes (MWCNTs) by one-step hydrothermal methods. The as-prepared Fe₃O₄-MWCNTs@Hemin nanocomposites exhibited excellent peroxidase-like activities in the activation of H₂O₂. The mechanisms, kinetics, and catalytic performances of Fe₃O₄-MWCNTs@Hemin were systematically studied. Fe₃O₄-MWCNTs@Hemin can oxidize dopamine (DA) to dopaquinone in the presence of H₂O₂, and the intermediate products dopaquinone can further react with β-naphthol to generate a highly fluorescent derivative at 415 nm excitation wavelength. Therefore, an innovative fluorescence platform for the detection of DA was developed. The fluorescence intensity increased linearly with DA concentration in the range 0.33 to 107 μM, with a low detection limit of 0.14 μM. Due to the excellent activity, substrate universality, fast response, high selectivity, and sensitivity of Fe₃O₄-MWCNTs@Hemin, the proposed fluorescence method was used to analyze complex biological blood samples with a satisfactory result. It demonstrated the significant potential for developing effective and dependable fluorescent analytical platforms for preserving human health. Graphical abstract