Development of conditions allowing restoration of hemopoiesis by allogeneic purified and in vitro multiplied pluripotent hemopoietic stem cells

Objectives of the project are: to study the effect of haemopoietic growth factors in vivo and in vitro; to investigate non-toxic conditioning regimens for bone marrow transplantation; to develop methods to identify and isolate hemopoietic stem cells. Preclinical studies were carried out in total body exposed rhesus monkeys to study whether treatment with haemopoietic growth factors can shorten the period of profound pancytopenia and/or immunodeficiency after transplantation of a limited number of bone marrow cells, or without bone marrow transplantation. It is also investigated as to whether the response to administration of growth factors can be used as a prognostic indicator of haemopoietic damage. (R.P.) 10 refs