Published 1985
| Version v1
Journal article
Measurement of mRNA concentration and mRNA half-life as a function of hormonal treatment
Creators
Description
The paper discusses the use of molecular hybridization techniques to measure mRNA concentrations in cells and tissues that are particularly useful to the analysis of small amounts of tissue. Two methods are presented for the quantitation of casein mRNA levels: (1) cDNA-excess liquid hybridization; and (2) the dot blot assay. The methods are discussed with some specific examples on the hormonal regulation of casein mRNA in the mammary gland explants. Experimental details are given for the isolation of RNA, DNA labelling techniques, RNA labelling techniques, and molecular hybridization techniques
Additional details
Publishing Information
- Journal Title
- Methods Enzymol.
- Journal Volume
- 109
- Series
- Methods Enzymol.
- Journal Page Range
- 572-592
- ISSN
- 0076-6879
- CODEN
- MENZA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18051044
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- ANIMAL CELLS; BIOCHEMICAL REACTION KINETICS; BIOLOGICAL HALF-LIFE; CASEIN; DNA; EXPERIMENTAL DATA; HORMONES; HYBRIDIZATION; LABELLED COMPOUNDS; LABELLING; MAMMARY GLANDS; MEASURING METHODS; MESSENGER-RNA; PHOSPHORUS 32; RESPONSE MODIFYING FACTORS; RNA; TRACER TECHNIQUES; TRITIUM COMPOUNDS
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DATA; DAYS LIVING RADIOISOTOPES; GLANDS; HYDROGEN COMPOUNDS; INFORMATION; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; NUMERICAL DATA; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; REACTION KINETICS