Published January 1981
| Version v1
Journal article
A simple radioenzymatic method to measure picogram amounts of DOPA in brain and biological fluids
Description
A simple radioenzymatic method for the determination of DOPA is described. The method is based on the conversion of DOPA to 3-O-[methyl-3H]DOPA by catechol-O-methyltransferase in the presence of S-adenosyl-[methyl-3H]methionine and purification of the labelled product by Sephadex G10 and Dowex 50 W x 4 ion exchange resin. The method has been applied to the assay of endogenous DOPA in different brain areas and to measuring DOPA accumulation after inhibition of aromatic amino acid DOPA decarboxylase. (author)
Additional details
Publishing Information
- Journal Title
- J. Neurochem.
- Journal Volume
- 36
- Journal Issue
- 1
- Series
- J. Neurochem.
- Journal Page Range
- 290-292
- ISSN
- 0022-3042
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- United Kingdom
- INIS RN
- 12629430
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BRAIN; DOPA; LABELLED COMPOUNDS; METHIONINE; QUANTITATIVE CHEMICAL ANALYSIS; RADIOENZYMATIC ASSAY; TRANSFERASES; TRITIUM
- Descriptors DEC
- AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CHEMICAL ANALYSIS; ENZYMES; HYDROGEN ISOTOPES; HYDROXY ACIDS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES