Published May 2022 | Version v1
Journal article

Research on the effect of Deinococcus radiodurans transformed by dsrA-flr-2 double gene on the enrichment performance of uranium(VI)

  • 1. University of South China, Hengyang (China). School of Public Health
  • 2. University of South China, Hengyang (China). School of Chemistry and Chemical Engineering
  • 3. University of South China, Hengyang (China). School of Resources Environment and Safety Engineering

Description

Strong-reducing gene (dsrA) and the fluorine-resistant gene (flr-2) were cloned into D. radiodurans to prepare a recombinant Deino-dsrA-flr-2 strains. Then, the influencing factors of uranium enrichment by Deino-dsrA-flr-2 and its enrichment mechanism were studied. When the initial concentration of uranium was 30 mg/L, the pH was 5, the biomass was 0.016 g and the enrichment time was 75 min, the Deino-dsrA-flr-2 had the best remove effeciency of U(VI). SEM and TEM could clearly observe uranium deposits on the surface of bacteria; EDS also indicated that uranium appeared on the surface of Deino-dsrA-flr-2. Compared with D. radiodurans, Deino-dsrA-flr-2 can better remove uranium. (author)

Additional details

Publishing Information

Journal Title
Journal of Radioanalytical and Nuclear Chemistry
Journal Volume
331
Journal Issue
5
Journal Page Range
p. 2195-2207
ISSN
0236-5731
CODEN
JRNCDM

Optional Information

Notes
43 refs.