Ecotoxicological assessment of micropollutant Diclofenac biosorption on magnetic sawdust: Phyto, Microbial and Fish toxicity studies

Highlights: • Diclofenac removal by low cost magnetized sawdust was reported for first time. • Characterization of MSD by various microscopic and spectroscopic techniques. • Electrostatic, π-π stacking and H- bonding interactions enhances adsorption capacity. • Ecotoxicological assessment viz. Phyto, Microbial and Fish toxicity of DCF were evaluated. Diclofenac (DCF), a persistent pharmaceutical micropollutant which occurs in the ecosystems causing adverse effects on aquatic as well as terrestrial organisms. In this study, magnetic sawdust (MSD) was prepared using co-precipitation method for biosorptive removal of DCF from water. The MSD was characterized using various analytical techniques like microscopic and spectroscopic analysis. Magnetometer study confirms the ferromagnetic behavior of the biosorbent which is a key advantage in the separation of MSD after biosorption. The effect of experimental parameters was optimized in batch mode with evaluated maximum efficiency of 86.12 % at pH 6, biosorbent dosage 25 mg for 50 mg/L of DCF. Ecotoxicological assessment has been performed for the treated and untreated sample using plant seeds, microbes and zebra fish to check the adverse effects of DCF on these organisms. Evaluation of toxicity studies revealed that inhibition concentration of DCF for various seeds (60.91 mg/L to 43.11 mg/L), E. coli (48.82 μg/mL) and B. subtilis (31.55 μg/mL). The lethal concentration of DCF on the Danio rerio was found to be 156.99 mg/L. In contrast, significant increase in both the concentration measures of DCF after biosorption was observed making this biosorbent a potent alternative to other available treatment measures.