Determination of endogenous formaldehyde level in human blood and urine by dimedone-14C radiometric method

A radiometric method was developed for the analysis of endogenous formaldehyde in human blood and urine using dimedone-14C reagent. The method is based on the fact that dimedone (5,5-dimethyl-cyclohexane-1,3-dione) can very easily condense with formaldehyde and other aldehydes. With the help of this radiometric method a sensitive technique and a more accurate evaluation is possible. By this method it is established that the formaldehyde level in the blood varies between 0.4-0.6 μg ml-1 and in urine 2.5-4.0 μg ml-1. The error of the determination and the chemical bonds of formaldehyde in the biological fluids are discussed. (author)