Published October 1979 | Version v1
Report Restricted

Cellular heredity in haploid cultures of somatic cells. Progress report, August 1978-September 1979

Creators

Description

During the past year we have continued our studies of the relation of ultraviolet mutagenesis to DNA repair in cultures of the haploid frog cell line ICR 2A. Our method of irradiation of cells in suspension was improved by construction of an improved detector with major sensitivity to the 254 nm Hg resonance line, to give better estimates of actual exposure of the cells. Using this method, dose-response and dose-fractionation studies on irradiation of ouabain resistance were carried out. The uv induction of this phenotype in the ICR 2A cell line was found to be less than that necessary for adequate analysis of dose-response curves. Cell fusion experiments using frog and mouse cells revealed an enhancement of mutagenesis in the mouse parent that will be explored in further work

Availability note (English)

MF available from INIS under the Report Number; Available from NTIS., PC A02/MF A01.

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Additional details

Additional titles

Augmented title (English)
Correlation between uv mutagenesis and DNA repair in cultured haploid frog cells (ICR 2A)

Publishing Information

Imprint Pagination
10 p.
Report number
COO--3110-31

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
11521760
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Numerical Data, Progress Report
Descriptors DEI
ANIMAL CELLS; BIOLOGICAL REPAIR; CELL CULTURES; CORRELATIONS; DNA; DOSE-RESPONSE RELATIONSHIPS; EXPERIMENTAL DATA; FRACTIONATED IRRADIATION; FROGS; GENETIC RADIATION EFFECTS; IN VITRO; ISOLATED VALUES; MUTAGENESIS; MUTATIONS; STRAND BREAKS; ULTRAVIOLET RADIATION
Descriptors DEC
AMPHIBIANS; ANIMALS; AQUATIC ORGANISMS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; DATA; DATA FORMS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; INFORMATION; IRRADIATION; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; VERTEBRATES