The analysis of differential rxpression of glycyrrhiza uralensis root gene by nitrogen ions implantationbased on cDNA-AFLP method

In order to study the differential expression of gene in licorice root induced by N⁺ implantation, cDNA-AFLP approach was employed. Total RNA was isolated from root of licorice. synthesizing the cDNA double chain by reverse transcription, using the PstI and MseI enzymes to digest the double standed cDNA, connecting the joint, pre-amplification, selective amplification. 286 differential expression cDNA fragments were obtained with 81 selective amplification primer pairs combinations. Which accounted for 20% of the total 1576 bands. The differential cDNA fragments were eluted, cloned and sequenced. 92 fragments of cDNA were obtained, 53 pieces of which retrieved high-homology DNA or protein mRNA sequences with known function by BLAST analysis. Functional alaysis indicated that some cDNA fragments might participate in metabolism pathways, signal transduction, stress responses etc. 30 fragments were showed homolory to unknown and hypothetical function genes. (authors)