Published 1978 | Version v1
Journal article

The radioimmunoassay of oxytocin in human plasma

  • 1. Brussels Univ. (Belgium)

Description

A detailed description is given of a technique for the radioimmunoassay of oxytocin in unextracted human plasma. Synthetic oxytocin was first 125I-iodinated and then purified by column chromatography. Antibodies to oxytocin were produced in rabbits. Oxytocinase activity in late pregnancy plasma was inhibited by the addition of o-phenantrolin. The method could detect 10μU oxytocin/ml. No assayable hormone was detected in plasma samples from men or non-pregnant women. Oxytocin levels ranging from 15 to 100 μU/ml could be detected in plasma samples taken during pregnancy and labour. (U.K.)

Additional details

Publishing Information

Journal Title
Int. J. Nucl. Med. Biol.
Journal Volume
5
Journal Issue
2-3
Series
Int. J. Nucl. Med. Biol.
Journal Page Range
144-147
ISSN
0047-0740