Published September 1993 | Version v1
Book Metadata-only

Detection and identification of tissue specific lectins of the tsetse fly, Glossina tachinoides: Midgut lectin activity with lipopolysaccharide binding specificity

Creators

  • 1. Charles Univ., Prague (Czechoslovakia). Dept. of Parasitology
  • 2. Czechoslovak Academy of Sciences, Ceske Budejovice (Czechoslovakia). Inst. of Parasitology

Description

Lectin that agglutinates human and animal red blood cells (RBCs) was demonstrated in midgut extracts of Glossina tachinoides. The highest haemagglutination titres were against pig and rabbit RBCs. Treatment of rabbit RBCs with pronase, trypsin, neuraminidase, bromelain, glutaraldehyde and periodate reduced the agglutination titres. The lectin is specific for amino, methyl and deoxy derivates of glucose, amino and methyl derivates of mannose, D-galactosamine, N-acetylneuraminic acid and trehalose. In addition, very high reactivity against the lipopolysaccharide of E. coli K 235 was found. Lectin is secreted to the midgut lumen. It consists of a 27 kilodalton protein component that is not glycosylated. Sandwich ELISA permits quantification of lectin in tissue samples. (author). 15 refs, 3 figs, 1 tab

Part of:
Management of insect pests: Nuclear and related molecular and genetic techniques

Additional details

Publishing Information

Publisher
IAEA.
Imprint Place
Vienna (Austria)
Imprint Title
Management of insect pests: Nuclear and related molecular and genetic techniques
Imprint Pagination
469 p.
Series
Proceedings series.
Journal Page Range
p. 557-565.
ISSN
0074-1884

Conference

Title
Nuclear and related molecular and genetic techniques.
Acronym
International symposium on management of insect pests
Dates
19-23 Oct 1992.
Place
Vienna (Austria).

INIS

Country of Publication
Austria
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
25014578
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference, Numerical Data
Descriptors DEI
BIOASSAY; ENZYME IMMUNOASSAY; EXPERIMENTAL DATA; GLOSSINA; HEMAGGLUTININS; INHIBITION; LECTINS
Descriptors DEC
AGGLUTININS; ANIMALS; ARTHROPODS; DATA; DIPTERA; FLIES; IMMUNOASSAY; INFORMATION; INSECTS; INVERTEBRATES; NUMERICAL DATA; PERFORMANCE TESTING; TESTING

Optional Information

Contract/Grant/Project number
Contract IAEA-R-6804/RB
Secondary number(s)
IAEA-SM--327/53.