Radiosynthesis, chromatographic evaluation and biodistribution of [¹²⁵I]iododobutamine as a radiotracer for myocardial perfusion imaging

The objective of this work was to develop a potential selective radiotracer for the non-invasive assessment of heart imaging. [¹²⁵I]iododobutamine was obtained via direct electrophilic substitution reaction with a high labeling yield of 96.79 ± 0.86%. The in vivo biodistribution of iodinated dobutamine showed high heart uptake of 31.49 ± 1.3%ID/g at 2 min after injection with low lungs and liver consumption which alleviate rapid myocardial imaging with significant minimum radiation hazards risk to the patients. Receptor blocking study showed reduction of heart uptake to 0.9 ± 0.01% which confirm the selectivity and specificity of [¹²⁵I]iododobutamine for beta-adrenoceptors. (author)