Isolation, structure determination and biological activity of 25-epi, 3α-carboxyacetylquercinic acid in daedalea dickinsii

In order to develope bioactive substances, dried fruit body of Daedalea dickinsii collected from Taeback mountain was extracted with ethanol. The compounds 1 and 2 were purified by solvent extraction, silica gel column chromatography and recrystallization from the ethanol extracts. Melting point, molecular weight and molar extinction coefficient of the compound 1 were estimated to be 167∼168 .deg. C, 572 and 5,040 at 208 nm. The structure of this compound 1 has been elucidated to be 24S, 25S, 3α-carboxyacetylquercinic acid using spectroscopic properties. This compound showed antibiotic activities against fungi, yeasts and bacteria and it also showed anticancer activity(IC50=64.5 μM/ml) against Korean stomach cancer cell. Melting point, molecular weight and molar extinction coefficient of the compound 2 were estimated to be 233∼235 .deg. C, 572 and 5,080 at 208 nm. The structure of this compound was elucidated to be 24S, 25R, 3α-carboxyacetylquercinic acid. The compound 2 showed different antibiotic activities with the compound 1