Electrospray ionization mass spectrometry with wooden tips: A review

Highlights: • Technical aspects of wooden-tip ESI-MS. • Features of wooden-tip ESI-MS that are different from those of conventional ESI-MS. • Important applications of wooden-tip ESI-MS. • Perspectives of wooden-tip ESI-MS. Electrospray ionization (ESI) is a powerful ionization technique in mass spectrometry (MS). There has been an increasing interest for the new development of ESI technique to extend its applications. ESI-MS with wooden tips (wooden-tip ESI-MS), an ESI technique invented in 2011, enabled not only new applications but also new insights into the ESI mechanism. In this review, the technical aspects of wooden-tip ESI-MS are described, the new features of wooden-tip ESI-MS for sampling and ionization of analytes are highlighted, and the important applications of wooden-tip ESI-MS in various fields in the past 10 years, including food safety, forensic investigation, environmental analysis, biomedical analysis and protein study, are summarized. The perspectives on the further development and applications of wooden-tip ESI-MS are also discussed.