Copper resorption in isolated rat hepatocytes
Creators
- 1. Kernforschungszentrum Karlsruhe G.m.b.H. (Germany, F.R.). Inst. fuer Kernphysik
- 2. Freiburg Univ. (Germany, F.R.). Medizinische Klinik 2
- 3. Freiburg Univ. (Germany, F.R.). Inst. fuer Organische Chemie und Biochemie
Description
Our aim is to investigate the Cu-uptake by isolated rat hepatocytes in an in-vitro experiment. Hepatocytes are cultured on foils to form cellular monolayers, which are exposed to CuSO4 solution. The trace elements P, S, Cl, K, Ca, Fe, Cu, Zn and Br are determined by PIXE, sweeping the proton microbeam in two dimensions across selected regions of the cell cultures. The concentration averages over positions covering the interior of hepatocytes or the intercellular gaps are formed and the behaviour of the various trace elements is studied as a function of the copper solution exposure time. In most cases cell nuclei are identified and evaluated separately. (orig./MG)
Abstract (German)
Unser Ziel ist es, die Cu-Aufnahme in isolierten Rattenhepatozyten in einem Laborexperiment zu untersuchen. Dazu werden Leberzellen auf Folien in einer einlagigen Schicht gezuechtet und einer Kupfersulfat-Loesung ausgesetzt. Die Spurenelemente P, S, Cl, K, Ca, Fe, Cu, Zn und Br werden mit Protonen-induzierter Roentgenstrahlung (PIXE) bestimmt, wobei der anregende Protonen-Mikrostrahl zeilenfoermig ueber ausgewaehlte Bereiche der Zellkulturen bewegt wird. Die Konzentrationen werden ueber alle Messpositionen gemittelt, die das Innere der Leberzellen oder die Zellzwischenraeume ueberdecken. Untersucht wird das Verhalten der verschiedenen Spurenelemente in Abhaengigkeit der Zeit, waehrend der die Probe der Kupfer-Sulfat-Loesung ausgesetzt wird. In den meisten Faellen koennen die Zellkerne identifiziert und gesondert ausgewertet werden. (orig./MG)
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Additional details
Publishing Information
- Imprint Pagination
- 26 p.
- Report number
- KFK--4428
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 19088132
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- CELL CULTURES; CELL NUCLEI; COPPER; COPPER SULFATES; COPPER 64; IN VITRO; LIVER CELLS; PIXE ANALYSIS; RATS; TIME DEPENDENCE; TRACE AMOUNTS; TRACER TECHNIQUES; UPTAKE
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CELL CONSTITUENTS; CHEMICAL ANALYSIS; COPPER COMPOUNDS; COPPER ISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; METALS; NONDESTRUCTIVE ANALYSIS; NUCLEI; ODD-ODD NUCLEI; OXYGEN COMPOUNDS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; SULFATES; SULFUR COMPOUNDS; TRANSITION ELEMENT COMPOUNDS; TRANSITION ELEMENTS; VERTEBRATES; X-RAY EMISSION ANALYSIS