A smartphone-based platform for point-of-use determination of alkaline phosphatase as an indicator of water eutrophication

A smartphone-based detection platform for the determination of alkaline phosphatase (ALP) is described. The method is based on the rational design of the stimulus–response of 7-methoxycoumarin-3-carboxylic acid (7-MC-3-COOH)–functionalized Eu-AMP infinite coordination polymer (ICP) nanoparticles. The blue fluorescence of 7-MC-3-COOH at 403 nm was suppressed, while the red fluorescence of Eu³⁺ at 615 nm was sensitized after the formation of 7-MC-3-COOH@Eu-AMP ICP. Upon exposure to ALP, the dephosphorylation of AMP resulted in the destruction of 7-MC-3-COOH@Eu-AMP ICP, and thereby, the blue fluorescence of 7-MC-3-COOH recovered; in the meantime, the sensitized red fluorescence was quenched. With the fluorescence intensity ratio F₆₁₅/F₄₃₀ as the signal readout, ALP can be detected within a concentration range 0.001 to 0.15 U mL⁻¹, and the limit of detection (LOD) was 0.00035 U mL⁻¹. Moreover, fluorescence color changes from red to blue could also be recognized by a portable device with the smartphone as a signal reader, and direct point-of-use testing (POUT) for ALP within a concentration range 0.005 to 0.7 U mL⁻¹ could be realized, with LOD of 0.0015 U mL⁻¹. Endowed with high sensitivity and superior reliability, the assay enabled direct monitoring of P-related water eutrophication in a freshwater lake with ALP as an indicator.