Optical Clearing of Human Skin Using Some Monosaccharides in vivo

We present the results of in vivo optical immersion clearing of human skin by aqueous solutions of some immersion agents (ribose, glucose, and fructose monosaccharides and glycerol), obtained using optical coherence tomography (OCT). To assess the efficiency of optical clearing, we determined the values of the rate of change of the light scattering coefficient, obtained using the averaged A-scan of the OCT signal in the derma section at a depth of 350–700 μm. A good correlation was observed between the rate of change of the light scattering coefficient and the potential of the optical clearing. Using complex molecular simulation of the interaction of a number of immersion clearing agents with collagen mimetic peptide (GPH)₃ using classical molecular dynamics and quantum chemistry, we found correlations between the efficiency of optical clearing and the energy of intermolecular interaction of cleaning agents with a fragment of collagen peptide.