Intensified simultaneous nitrification and denitrification performance in integrated packed bed bioreactors using PHBV with different dosing methods

To explore an effective approach of simultaneous nitrification and denitrification in wastewater with low C/N ratios, integrated packed bed bioreactors based on poly(3-hydroxybutyrate-hydroxyvalerate) (PHBV) with different dosing methods were designed. The removal efficiency of NH₄⁺-N in bioreactor with aeration was 88.62%, and higher NO₃⁻-N removal efficiency was observed in bioreactor filled with grainy PHBV (95.21%) than bioreactor filled with strip PHBV (93.34%). Microbial study indicated that microbes harboring amoA and nirS genes preferred to attach on the surface of ceramsite, and significant differences in microbial community compositions at phylum and genus levels were observed. To summarize, it is feasible to utilize grainy PHBV for simultaneous and efficient removal of NH₄⁺-N and NO₃⁻-N from wastewater with low C/N ratios.