Development of the detection of synthetic corticosteroids from a urinary matrix by high-performance liquid chromatography coupled with mass spectrometry

Within the framework of the struggle against doping in sports, this academic engineering report first presents the French National Doping Detection Laboratory, forbidden substances and laboratory's activities, and then corticosteroids and their actions on metabolism (glucocorticoids, synthetic glucocorticoids in sport), analytical techniques (immuno-dosing, chromatographic methods). The author then reports the theoretical part of the study: instrumentation, ionisation techniques, analysers, sample preparation), and the experimental part of the study (standards and solvents, optimisation of chromatographic conditions, optimisation of spectrometric conditions for screening search, preparation in solid phase extraction, method verification by using an enriched urine, confirmation stage, applications and perspectives).