Fundamental study of Triosorb M-125

The usefullness of the Triosorb M test for measuring thyroid function indirectly was evaluated in 82 pregnant women and subjects with or without thyroid abnormalities. Satisfactory reproducibility was obtained in intraassays and interassays; coefficient variation were +-1.71% and +-2.44% respectively. Triosorb M values were affected by changes of incubation time and temperature, especially by the latter. Triosorb M values dropped significantly in hyperthyroid sera as the incubation temperature rose, while values rose slightly in hypothyroid sera. Incubation time had almost the same effect on both hyper- and hypothyroid sera values. A good correlation was observed between Triosorb M values and Triosorb values, when incubated at 200C or 250C for 20 min. Incubated at 250C, Triosorb M values were lower than those of Triosorb tests on the same hyperthyroid sera. Incubated at 200C, both tests showed very similar values in hyperthyroid sera as well as in hypothyroid and normal sera. In normal subjects, Triosorb M values were 27.9+-2.3% with a rare overlap with those of hyperthyroid, and a moderate overlap with those of hypothyroid. The Triosorb M test requires only 0.5 ml of serum and can be carried on in a short time with strict controlling of the incubation temperature and time, and might be one of the useful examinations of thyroid function. (auth.)