Published 1983 | Version v1
Journal article Metadata-only

Visualization of different types of hapten-cell membrane interaction by fluorine high field nuclear magnetic resonance

Creators

  • 1. INSERM U95, 54 - Vandoeuvre (France)
  • 2. CRBPCC-ERA-CNRS No 315, Faculte des Sciences, 44 - Nantes (France)
  • 3. Institut Armand-Frappier, Laval-des-Rapides (Canada)

Description

Using a high field spectrometer (5.9 Tesla) 19F-NMR spectrum of soluble material from 4 trifluoromethyl 2,6 dinitrobenzene sulphonate (CF3-DNBS) treated murine lymphocytes was recorded. CF3-DNBS is a fluorinated analog of 2,4,6 trinitrobenzene sulphonate (TNBS), and these compounds have been found to create cross-reacting antigenic modifications of cell surface. At least 4 distinguishable signals have been detected, and 19F-NMR could be used to study, at a molecular level, some immunochemical problems concerning modification with TNBS

Abstract (French)

Utilisant un spectrometre RMN a haut-champ (5,9 Tesla) on a obtenu des spectres RMN 19F de lymphocytes de souris traites par le 4 trifluoromethyl 2,6 dinitrobenzene sulfonate (CF3-DNBS). CF3-DNBS est un analogue fluore du 2,4,6 trinitrobenzene sulfonate (TNBS) et ces composes ont provoque des modifications antigeniques de la surface cellulaire. Au moins 4 signaux ont ete mis en evidence et le spectre RMN 19F pourrait etre utilise pour etudier, au niveau moleculaire, des problemes immuno-chimiques concernant les modifications avec TNBS

Additional details

Additional titles

Original title (French)
Visualisation, par resonance magnetique nucleaire du fluor a haut-champ, de differents types d'interaction haptene-membrane cellulaire

Publishing Information

Journal Title
Biol. Cell.
Journal Volume
46
Journal Issue
1
Series
Biol. Cell.
Journal Page Range
91-94
ISSN
0399-0311

INIS

Country of Publication
France
Country of Input or Organization
France
INIS RN
15033424
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ANTIGENS; CELL MEMBRANES; FLUORINE 19; LYMPHOCYTES; NUCLEAR MAGNETIC RESONANCE; PICRIC ACID
Descriptors DEC
ANIMAL CELLS; AROMATICS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; EXPLOSIVES; FLUORINE ISOTOPES; HYDROXY COMPOUNDS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; MAGNETIC RESONANCE; MATERIALS; MEMBRANES; NITRO COMPOUNDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHENOLS; RESONANCE; SOMATIC CELLS; STABLE ISOTOPES