Published July 2009 | Version v1
Miscellaneous Metadata-only

Inheritance of Yellow Vein Mosaic Disease Resistance, a Mutant Phenotype from Gamma Radiation Mutagenesis in Okra

Creators

  • 1. Thailand Institute of Nuclear Technology, Public Organization, Bangkok (Thailand)
  • 2. Horticultural Research Institute, Department of Agriculture, Bangkok (Thailand)
  • 3. Crop Protection Research and Development Office, Department of Agriculture, Bangkok (Thailand)

Description

Full text: The yellow vein mosaic disease (YVMD) is one of the major diseases affecting export-grade okra production in Thailand. YVMD-resistant B4610 mutant was generated through gamma irradiation of the Okura variety of okra. This study aimed at determining the inheritance of YVMD resistance from B4610 through genetic analysis. Segregation analysis of the F2 and BC1F1 progeny from the cross between B4610 and Pichit 03, a susceptible variety, indicated that YVMD resistance phenotype resulted from a single locus mutation (p > 0.05). In addition, a polymerase chain reaction (PCR) assay was developed to aid in the assignment of YVMD resistance phenotype. Combinations of primers AV1F1 or AV1F2 with AV1R1 successfully amplified a DNA fragment from infected susceptible individuals but not from resistant individuals. DNA sequencing of the PCR product confirmed the identity of AV1 gene belonging to the yellow vein mosaic virus

Availability note (English)

Available in abstract form only, full text available from Thailand Institute of Nuclear Technology, Public Organization, Nakorn Nayok (Thailand)
Part of:
Nuclear Science and Technology for Thai Society

Additional details

Publishing Information

Imprint Pagination
1 p.
Report number
INIS-TH--265

Conference

Title
11. Conference on Nuclear Science and Technology
Dates
2-3 Jul 2009
Place
Bangkok (Thailand)

INIS

Country of Publication
Thailand
Country of Input or Organization
Thailand
INIS RN
43050903
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference, Numerical Data, Non-conventional Literature
Descriptors DEI
COBALT 60; DOSES; EXPERIMENTAL DATA; HERBS; IRRADIATION; MICROORGANISMS; PLANTS
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; COBALT ISOTOPES; DATA; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUMERICAL DATA; ODD-ODD NUCLEI; PLANTS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES

Optional Information