Published November 1996
| Version v1
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Production of monoclonal antibodies for use in immunoassays based on the magnetizable solid phase separation technique
- 1. National Inst. of Health (Thailand). Dept. of Medical Sciences
Description
Monoclonal antibodies to TSH were produced by using mouse-ascites techniques. Various methods for purifying the antibody from the ascetic fluid have been tried in order to obtain an appropriate TSH kit production protocol. The purified antibodies were then immobilized on magnetizable cellulose for developing an IRMA for TSH. A detailed study of the assay system, including the stability of the magnetic adsorbent was made, which showed that the SCIPAc magnetizable cellulose is suitable for the production of TSH - Blood spot IRMA kits for use in the Neonatal hypothyroid screening programme to be launched in Thailand in the near future. (author). 4 refs, 12 figs, 2 tabs
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Additional details
Publishing Information
- Imprint Title
- Antibodies immobilized on magnetic particles for radioimmunoassay and immunoradiometric assay of hormones. Final report of a co-ordinated research programme 1991-1995
- Imprint Pagination
- 107 p.
- Journal Page Range
- p. 95-105.
- ISSN
- 1011-4289
- Report number
- IAEA-TECDOC--914
INIS
- Country of Publication
- International Atomic Energy Agency (IAEA)
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 28023516
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ANTIBODIES; DIAGNOSTIC USES; HORMONES; HYPOTHYROIDISM; MONOCLONAL ANTIBODIES; RADIOIMMUNOASSAY; RADIOIMMUNODETECTION; SAMPLE PREPARATION; TSH
- Descriptors DEC
- DIAGNOSTIC TECHNIQUES; DISEASES; ENDOCRINE DISEASES; IMMUNOASSAY; ISOTOPE APPLICATIONS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOASSAY; TRACER TECHNIQUES; USES