Published November 2019 | Version v1
Journal article

Assay of 1-hydroxypyrene via aggregation-induced quenching of the fluorescence of protamine-modified gold nanoclusters and 9-hydroxyphenanthrene-based sensitization

  • 1. University of South China, College of Public Health (China)

Description

This work describes a method for the determination of 1-hydroxypyrene (OH-Py) via aggregation-induced quenching of the emission of protamine-coated gold nanoclusters using 9-hydroxyphenanthrene (OH-Phe) as a sensitizer to boost the emission efficiency of nanoprobe. Under optimum conditions, the drop in fluorescence intensity at excitation/emission wavelengths of 300/596 nm is proportional to the concentrations of OH-Py in the range from 1.0 to 65 nM. The relative standard deviations are 4.2, 2.4 and 1.9% (for n = 11) at concentration levels of 8.0, 32 and 48 nM of OH-Py, respectively. The detection limit is 0.3 nM which is much lower than that of some previously reported methods. The recoveries from urine samples spiked with OH-Py ranged between 94.4 and 98.8%.

.

Additional details

Identifiers

Publishing Information

Journal Title
Mikrochimica Acta
Journal Volume
186
Journal Issue
11
Journal Page Range
p. 1-9
ISSN
0026-3672
CODEN
MIACAQ

Optional Information

Copyright
Copyright (c) 2019 Springer-Verlag GmbH Austria, part of Springer Nature