Synthesize and preliminary biodistribution of 99Tcm(CO)3-PNP5

99Tcm (CO)3-PNP5 (PNP5: bis (dimethoxypropylphosphinoethyl) ethoxyethyl- amine) is synthesized through a simple two-step procedure by ligand exchange reaction and its biological characters are studied. Labelling conditions of 99Tcm (CO)3-PNP5 are optimized. Its labelling yield and radio chemical purity are all over 90% determined by TLC. The results of partition coefficient, charge character and stability studies indicate that 99Tcm (CO)3-PNP5 is a lipophilic cation ligand with complex with positiue charge and good stability. Biological properties of 99Tcm (CO)3-PNP5 and 99Tcm (CO)3-PNP5 (T) (adding Tween) are valued contrastively in mice. The results show that 99Tcm(CO)3-PNP5(T) have higher myocardial uptake, lower liver uptake, and higher heart-to-liver ratio. It indicats that the biological properties of 99Tcm (CO)3-PNP5 are improved obviously by adding Tween-80. (authors)