Incorporation of 3H-2,3-DL-valine into pancreatic proteins of adrenalectomized rats stimulated with pancreozymin-CCK and secretin

Incorporation of 3H-valine was investigated in bilaterally adrenalectomized rats stimulated with pancreozymin-CCK and secretin 14 days after operation. The growth of the rats, the blood pressure, the amount of pancreatic juice and the amylase output was less in adrenalectomized animals as compared with sham-operated or adrenalectomized and corticosterone-substituted controls. The amylase concentration in the pancreatic juice revealed no difference between the groups but the output of amylase remained below that of the controls. The incorporation of labelled valine was higher in the pancreatic tissue and proteins of adrenalectomized animals than in the controls. It is assumed that the adrenalectomy causes primarily a depletion in the metabolic activity and systemic blood pressure; diminished pancreatic juice secretion and output of amylase are only secondary consequences which are not compensable by hormonal stimulation of pancreatic secretion. (author)