Codon usage bias of Canarium album (Lour.) R. transcriptome and its influence factors

To explore codon usage bias (CUB) pattern of Canarium album (Lour.) R., the transcriptome of Fulan-1 was used to analyze CUB parameters by codonW and EMBOSS, and the possible formative factors were discussed. The results showed that, the effective number of codon (ENc), GC content (GC) and GC content on the 3 rd site (GC3s) is 51.80, 0.435 and 0.377 respectively, which indicate that CUB level of Canarium album (Lour.) R. is low, but it is biased towards the synonymous codons with A or T on the 3 rd site. The differences of relative synonymous codon usage (RSCU) between high-expression and low-expression gene sample groups are not significant, but CGC, ATC, CTC and ACC are defined as the major preference codons of Canarium album (Lour.) R.. Based on composition analysis, neutrality plot, ENc-GC3 s plot and PR2 plot, the CUB of Canarium album (Lour.) R. is mainly due to the effect of mutation pressure. In addition, Nicotiana sylvestris and Saccharomyces cerevisiae may be the suitable receptors for gene heterologous transgenosis of Canarium album (Lour.) R.. These results would provide scientific basis for further research of genetic background and molecular evolution of Canarium album (Lour.) R.. (authors)