Published July 25, 2006 | Version v1
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Coupling mechanisms between nucleosome assembly and the cellular response to DNA damage

Creators

Description

Cells are continuously exposed to genotoxic stresses that induce a variety of DNA lesions. To protect their genome, cells have specific pathways that orchestrate the detection, signaling and repair of DNA damages. This work is dedicated to the characterization of such pathways that couple the DNA damage response to the assembly of chromatin, a complex that protects and regulates DNA accessibility. We have focused our study on two multifunctional proteins: Rad53, a central checkpoint kinase in the cellular response to DNA damage and Asf1, a histone chaperone involved in chromatin assembly. We have characterized in vitro the binding mode of Asf1 with Rad53 and Asfl with histones. This study is associated with the functional analysis of the role of these interactions in vivo in yeast cells. (author)

Abstract (French)

Les cellules sont en permanence soumises a des stress genotoxiques induisant des dommages de leur ADN. Elles ont mis en place des mecanismes specifiques pour detecter ces dommages, signaler leur presence et les reparer. Ce travail a pour but de mieux comprendre les mecanismes de couplage entre la signalisation des dommages de l'ADN et l'assemblage de la chromatine (complexe proteique qui protege et regule l'accessibilite de l'ADN). Il s'interesse plus particulierement a deux proteines multifonctionnelles: la kinase Rad53, qui orchestre la reparation des dommages de l'ADN et la division cellulaire, et Asf1, un chaperon d'histones implique dans l'assemblage de la chromatine. Par une approche couplant biochimie et biologie structurale, nous avons caracterise in vitro le mode d'interaction entre Asf1 et les histones et, entre Asf1 et Rad53. Cette etude est associee a l'analyse du role fonctionnel de ces interactions in vivo dans les cellules de levure

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Additional details

Additional titles

Original title (French)
Couplages entre l'assemblage du nucleosome et la reponse cellulaire aux stress genotoxiques

Identifiers

URL
http://www.iaea.org/inis/Contacts/;

Publishing Information

Imprint Pagination
243 p.
Report number
FRCEA-TH--8732

INIS

Country of Publication
France
Country of Input or Organization
France
INIS RN
48072188
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Thesis
Descriptors DEI
CHROMATIN; DNA DAMAGES; DNA REPAIR; HISTONES; IN VITRO; IONIZING RADIATIONS; YEASTS
Descriptors DEC
BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; EUMYCOTA; FUNGI; MICROORGANISMS; ORGANIC COMPOUNDS; PLANTS; PROTEINS; RADIATIONS; REPAIR

Optional Information

Notes
191 refs.; Available from the INIS Liaison Officer for France, see the 'INIS contacts' section of the INIS-NKM website for current contact and E-mail addresses: http://www.iaea.org/inis/Contacts/; Also available from Bibliotheque Universitaire Pierre et Marie Curie, 4 Place Jussieu, 75005 Paris (France)